Understand that large amounts of human insulin can be manufactured from genetically modified bacteria that are grown in a fermenter
The transgenic bacterial cell has been transformed from normal bacterial cell by the addition of recombinant DNA (plasmid + human gene for insulin).
A culture of bacteria will be ingested into the fermentor.
In this fermentor chamber we need to consider:
- Nutrient --> Used to manufacture insulin protein
- Temperature --> Optimal temperature
- pH
- Gases
----> Increase in population, in which the bacteria will then switch on the gene for insulin and manufacture protein insulin.
It will then be necessary to remove the product and carry out purification
Purification = Downstream processing
Genetically engineered human insulin = Humulin
No comments:
Post a Comment